Plasmid Preparation:Article Title: CYRI-A limits invasive migration through macropinosome formation and integrin uptake regulation
Article Snippet: .. mApple-α-5-Integrin-12 (plasmid #54864; RRID:Addgene_54864; Addgene), mCherry-Clathrin LC-15 (plasmid #55019; RRID:Addgene_55019; Addgene), and mCherry-Rab5a-7 (plasmid #55126; RRID:Addgene_55126; Addgene) were gifts from Michael Davidson ( ); pcDNA3/hArf1(WT)-mCherry (plasmid #79419; RRID:Addgene_79419; Addgene) was a gift from Kazuhisa Nakayama ( ); CAV1-mCherry (plasmid #27705; RRID:Addgene_27705; Addgene) was a gift from Ari Helenius ( ); and PH-Btk-GFP (plasmid #51463; RRID:Addgene_51463; Addgene) was a gift from Tamas Balla ( ). .. Anti–CYRI-A antibody (rabbit, Sigma-Aldrich, not in production; Western blotting), anti–CYRI-B antibody (rabbit, #HPA009076, RRID:AB_1848402; Sigma-Aldrich; Western blotting), anti-GAPDH (mouse, #MAB374, RRID:AB_2107445; Millipore; Western blotting), anti-Tubulin DM1A (mouse, #T6199, RRID:AB_477583; Sigma-Aldrich; Western blotting), anti-GST (rabbit, #2622, RRID:AB_331670; CST; Western blotting), anti-GFP (mouse, #2955, RRID:AB_1196614; CST; Western blotting), Phalloidin 488 (#A12379; Molecular Probes), anti-Integrin β 1 antibody [12G10] (rabbit, #ab30394, RRID:AB_775726; Abcam; immunofluorescent and Western blotting), recombinant anti-Integrin α 5 antibody [EPR7854] (rabbit, #ab150361, RRID:AB_2631309; Abcam; immunofluorescent and Western blotting), anti-Integrin α5 (preservative free) antibody, clone SNAKA51 (mouse, #MABT201; Millipore; immunofluorescent, internalization assay, and flow cytometry), Alexa Fluor 488 anti-human CD29 antibody TS2/16 (mouse, #303015, RRID:AB_493026; BioLegend; flow cytometry), Alexa Fluor 488 anti-mouse CD49e Antibody (rat, #103810, RRID:AB_528839; BioLegend; flow cytometry), anti-MMP-14 antibody, catalytic domain, clone LEM-2/63.1 (mouse, #MAB3329, RRID:AB_570600; Millipore; flow cytometry), donkey anti-rabbit 680-nm (#A10043, AB_2534018; Invitrogen; Western blotting), donkey anti-mouse 680-nm (#A10038, AB_2534014; Invitrogen; Western blotting), goat anti-mouse 800-nm (#SA5-35521, AB_2556774; Thermo Fisher Scientific; Western blotting), and goat anti-rabbit 800-nm (#SA5-35571, AB_2556775; Thermo Fisher Scientific; Western blotting).
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